Autoclave Uses And Functions

November 3, 2021

An  autoclave  is a laboratory instrument that can also be owned by an individual and the manufacturer, in a laboratory this instrument is used to sterilize dangerous microorganisms and others.

The study and deepening of the Autoclave are important in all areas of health, for the prevention of infections in humans. For this there are various types of sterilization, which destroy the bacteria that are found, and are produced through the environment.
The exposed subject allows us to understand and better know the proper handling and importance of the use of the Autoclave, at the same time that it provides us with the necessary bases for its proper use.
An autoclave is a device used to sterilize medical or laboratory material, using steam at high pressure and temperature for this. The use of an inactivates all viruses and bacteria, although it has become known that some microorganisms, as well as prions, can withstand the temperatures of the autoclave. The Autoclave system is a strict application in hospitals in cleaning, disinfection and sterilization processes. It provides safety for patients and healthcare workers. These procedures are essential in the adequate control of intrahospital infections.

Role  of  Sterilization

It includes all the physical, mechanical and chemical procedures used to destroy, inactivate or retain germs in general and pathogens in particular. By means of this, laboratory materials, surgical elements and the patient’s skin reach a state of disinfection that avoids operative contamination. This is the complete elimination of all forms of microbial life. It can be achieved through chemical, physical and gaseous methods.
All items to be sterilized must be placed inside the tray, it must be able to take care that the elements, in case of boiling, do not fall and spill into the chamber; Make sure that the items you are going to sterilize are “autoclavable”, and pay special attention to dangerous liquids.
We recommend that the container be twice as large as the liquid it will store, this is to avoid spillage when the liquid is boiling inside the chamber, the lids must be loosened to avoid breaking the container, finally, you have to place the trash inside autoclave bags   and 5-inch trays.
The autoclave has the advantage of producing a rapid rise in temperature in short sterilization times and of not leaving toxic residues on the material.

How is autoclaved sterilization done?

The process is done in a short period without the application of reagents, in this way the cleanliness of the tools is ensured before reuse, such as equipment for hospitals, dental, etc. Therefore, an autoclave is an environmentally friendly option that helps reduce waste and encourages the reuse of materials.

It is the most effective method of sterilization, which consists of the application of wet or dry heat. Steam at 121 ° C (250 ° F) under pressure. Autoclaving is the most convenient method for fast and efficient sterilization

An autoclave is basically made up of a rigid and hermetic chamber that includes a door with safety devices to allow the objects to be sterilized to be inserted. This chamber has installed devices for measuring pressure and temperature, and heating elements to keep it warm.
The reason is to keep the camera at a temperature higher than that of the environment, thus avoiding undesirable changes. The most common cycles that manufacturers incorporate into autoclaves are:
  • 105 ºC for the disinfection of liquids and delicate objects. Below 120 ºC, one can only speak of disinfection, never sterilization.
  • 120 ºC, This is applied for the general sterilization of instruments, gloves and clinical tissues.
  • 134 ºC, In this the surgical or risk material is sterilized.
  • 143 ºC, Rapid cycle, to sterilize mainly instruments whose use is immediate or urgent.

Drying cycle, This period arises from the need to eliminate by evaporation at medium temperature the inevitable condensation of water at the end of the cycle. sterilize.

Hot air or dry sterilization: There are PASTEUR ovens that reach temperatures of 160 to 180 ° C and are used to sterilize glass syringes, instruments and surgical clothing, following these rules:  General surgical instruments (40 to 180 ° for 15 30 minutes), ophthalmic surgery instruments (140 ° for 30 minutes); Glass (140 ° for 20 minutes); clothes (150 – 160 ° for one hour).

The  autoclave  has a risk in use, it generates a lot of energy in the sterilization process, so there is a high risk of injury to the user, therefore, to avoid possible damage caused by the incorrect use of this instrument, follow steps below.

  • Wear an apron, gloves, and goggles to handle hot containers.
  • Be sure to turn it off and disconnect the cable before removing the materials from the chamber.
  • Allow the materials to cool before removing them from the autoclave sterilizer.
  • Carefully remove the materials from the autoclave.
Dr. Loony Davis5
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Born and raised in Brussels in an English family, I have always lived in a multicultural environment. After several work experiences in marketing and communication, I came to Smart Water Magazine, which I describe as the most exciting challenge of my career.
I am a person with great restlessness and curiosity to learn, discover what I do not know, as well as reinvent myself daily, someone who is curious about life and wants to know. I enjoy sharing knowledge.
This is my personal project but I also collaborate in other blogs, it is the case, the most important web on water currently exists in the US, if you are interested you can read my articles here.

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